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PLOD3 Antibody

Product Specifications

Background

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.

NCBI Gene ID

8985

Swiss Prot

O60568

Accession Number

O60568

Host

Rabbit

Reactivity

Human

Immunogen

This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=56-612

Calculated Molecular Weight

85 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PLOD3

NCBI Official Name

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

NCBI Organism

Homo sapiens

Background Reference 01

Wang, C., et al. J. Cell. Mol. Med. 13 (3) :508-521 (2009)

Background Reference 02

Salo, A.M., et al. Am. J. Hum. Genet. 83 (4) :495-503 (2008)

Background Reference 03

Salo, A.M., et al. J. Cell. Physiol. 207 (3) :644-653 (2006)

Background Reference 04

Wang, C., et al. Matrix Biol. 21 (7) :559-566 (2002)

Other Product Names

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3, Lysyl hydroxylase 3, LH3, PLOD3

Tested Applications

WB

Physical Properties

Liquid

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