SF3B3 Antibody

Product Specifications

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF (II) 31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF (II) -containing complex) . These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

NCBI Gene ID

23450

Swiss Prot

Q15393

Accession Number

Q15393

Host

Rabbit

Reactivity

Human

Immunogen

This SF3B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1046-1074 amino acids from the C-terminal region of human SF3B3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

136 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SF3B3

NCBI Official Name

Splicing factor 3B subunit 3

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 02

Will, C.L., et al. RNA 10 (6) :929-941 (2004)

Background Reference 03

Cavusoglu, N., et al. Proteomics 3 (2) :217-223 (2003)

Background Reference 04

Will, C.L., et al. EMBO J. 21 (18) :4978-4988 (2002)

Other Product Names

Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, SF3B3, KIAA0017, SAP130