NIACR1 Antibody

Product Specifications

Background

NIACR1 acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D) -beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G (i) -protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.

NCBI Gene ID

338442

Swiss Prot

Q8TDS4

Accession Number

Q8TDS4

Host

Rabbit

Reactivity

Human

Immunogen

This NIACR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human NIACR1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction, Growth Factors

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

42 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

HCAR2

NCBI Official Name

Hydroxycarboxylic acid receptor 2

NCBI Organism

Homo sapiens

Background Reference 01

Li, X., et al. Biochem. Pharmacol. 80 (9) :1450-1457 (2010)

Background Reference 02

Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

Background Reference 03

Li, G., et al. J. Biol. Chem. 285 (29) :22605-22618 (2010)

Background Reference 04

Mandrika, I., et al. Biochem. Biophys. Res. Commun. 395 (2) :281-287 (2010)

Other Product Names

Hydroxycarboxylic acid receptor 2, G-protein coupled receptor 109A, G-protein coupled receptor HM74A, Niacin receptor 1, Nicotinic acid receptor, HCAR2, GPR109A, HCA2, HM74A, NIACR1