BPGM Antibody

Product Specifications

Background

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

NCBI Gene ID

669

Swiss Prot

P07738

Accession Number

P07738

Host

Rabbit

Reactivity

Human

Immunogen

This BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the C-terminal region of human BPGM.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Monkey

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

BPGM

NCBI Official Name

Bisphosphoglycerate mutase

NCBI Organism

Homo sapiens

Background Reference 01

Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

Background Reference 02

Wang, Y., et al. J. Biol. Chem. 281 (51) :39642-39648 (2006)

Background Reference 03

Wang, Y., et al. J. Biol. Chem. 279 (37) :39132-39138 (2004)

Background Reference 04

Fujita, T., et al. J. Biochem. 124 (6) :1237-1244 (1998)

Other Product Names

Bisphosphoglycerate mutase, BPGM,3-bisphosphoglycerate mutase, erythrocyte,3-bisphosphoglycerate synthase,3-diphosphoglycerate mutase, DPGM, BPG-dependent PGAM, BPGM