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POLR1B Antibody

Product Specifications

Background

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]) .

NCBI Gene ID

84172

Swiss Prot

Q9H9Y6

Accession Number

Q9H9Y6

Host

Rabbit

Reactivity

Human

Immunogen

This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

128 kDa

Fragment

Rabbit Ig

Symbol

POLR1B

NCBI Official Name

DNA-directed RNA polymerase I subunit RPA2

NCBI Organism

Homo sapiens

Background Reference 01

Wen, L., et al. Biochem. Biophys. Res. Commun. 367 (4) :846-851 (2008)

Background Reference 02

Wu, C., et al. Proteomics 7 (11) :1775-1785 (2007)

Background Reference 03

Johnson, S.S., et al. Mol. Cell 26 (3) :367-379 (2007)

Background Reference 04

Panova, T.B., et al. Mol. Cell. Biol. 26 (16) :5957-5968 (2006)

Other Product Names

DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B

Tested Applications

WB, IHC-P, Flow

Protein ID

92090637

Physical Properties

Liquid

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