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TdT Antibody

Product Specifications

Background

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

NCBI Gene ID

1791

Swiss Prot

P04053

Accession Number

P04053

Host

Rabbit

Reactivity

Human

Immunogen

This TdT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human TdT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=55-915

Calculated Molecular Weight

59 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

DNTT

NCBI Official Name

DNA nucleotidylexotransferase

NCBI Organism

Homo sapiens

Background Reference 01

Kubota, T., et al. Genes Cells 12 (8) :941-959 (2007)

Background Reference 02

O'Malley, D.P., et al. Haematologica 91 (8) :1139-1140 (2006)

Background Reference 03

Grupe, A., et al. Am. J. Hum. Genet. 78 (1) :78-88 (2006)

Background Reference 04

Thai, T.H., et al. J. Immunol. 173 (6) :4009-4019 (2004)

Other Product Names

DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, DNTT, TDT

Tested Applications

WB

Protein ID

311033533

Physical Properties

Liquid

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