GAD1 Antibody

Product Specifications

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

NCBI Gene ID

2571

Swiss Prot

Q99259

Accession Number

Q99259

Host

Rabbit

Reactivity

Human

Immunogen

This GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514-543 amino acids from the C-terminal region of human GAD1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology, Obesity, Neuroscience, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

67 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

GAD1

NCBI Official Name

Glutamate decarboxylase 1

NCBI Organism

Homo sapiens

Background Reference 01

Lanoue, A.C., et al. Exp. Neurol. 226 (1) :207-217 (2010)

Background Reference 02

Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :

Background Reference 03

Terranova, C., et al. Alcohol 44 (5) :407-413 (2010)

Background Reference 04

Ruano, G., et al. Pharmacogenomics 11 (7) :959-971 (2010)

Other Product Names

Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD, GAD67

Tested Applications

Protein ID

1352213

Physical Properties

Liquid

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