Tau (phospho Ser416) Antibody

Product Specifications

Background

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985) . Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990) . Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995) . Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990) .

NCBI Gene ID

29477

Swiss Prot

P19332

Accession Number

P19332

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Chicken

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of rat tau.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Tau (Ser416) antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

59/65/68

Specificity

Specific for ~59, 65, 68k tau protein phosphorylated at Ser416.

Symbol

Mapt

NCBI Official Name

Microtubule-associated protein tau

NCBI Organism

Rattus norvegicus

Background Reference 01

Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Bio Oct; 101 (4) :1371-8.

Background Reference 02

Lee V.M.Y. (1995) Disruption of the cytoskeleton in Alzheimer’s disease. Curr. Opin. Neurobiol. 5, 663-668.

Background Reference 03

Goedert M. and Jakes R. (1990) Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization. EMBO J 9, 4225-4230.

Background Reference 04

Steiner B., Mandelkow E.M., Biernat J. et al. (1990) Phosphorylation of microtubule-associated protein tau: identification of the site for Ca2+-calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles. EMBO J. 9, 3539-3544.