Choline Acetyltransferase Antibody

Product Specifications

Background

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999) . Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993) . It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999) .

NCBI Gene ID

1103

Swiss Prot

P28329

Accession Number

P28329

Host

Goat

Reactivity

Human, Mouse, Rat, Goat, Chicken

Immunogen

Native choline acetyltransferase purified from human placenta.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Affinity Purified

Concentration

Batch dependent

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Choline Acetyltransferase antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Specificity

Specific for the ~ 70k choline acetyltransferase protein

Symbol

CHAT

NCBI Official Name

Choline O-acetyltransferase

NCBI Organism

Homo sapiens

Background Reference 01

Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.

Background Reference 02

Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94 (1) :21-31.

Background Reference 03

Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.