Neurofilament NF-M Antibody [3H11]

Product Specifications

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.

NCBI Gene ID

24588

Swiss Prot

P12839

Accession Number

P12839

Host

Mouse

Reactivity

Human, Mouse, Rat, Chicken

Immunogen

Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Clonality

Monoclonal

Clone

3H11

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Total IgG fraction

Concentration

Batch dependent

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Neurofilament NF-M antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

145

Fragment

IgG1

Specificity

Specific for the ~145k Neurofilament M protein.

Symbol

Nefm

NCBI Official Name

Neurofilament, medium polypeptide

NCBI Organism

Rattus norvegicus

Background Reference 01

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.

Background Reference 02

Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320 (3) :156-60.