PLK1 (phospho Thr210) Antibody

Product Specifications

Background

Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004) . Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005) . It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005) .

NCBI Gene ID

5347

Swiss Prot

P53350

Accession Number

P53350

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish, Bovine, Frog

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

PLK1 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, Xenopus and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Symbol

PLK1

NCBI Official Name

Polo-like kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH (2004) Polo-like kinase 1 is required for bipolar spindle formation but is dispesible for anaphase promoting complex cdc20 activation and initiation of cytokinesis. J. Biol. Chem. Aug 27;279 (35) :36841-54.

Background Reference 02

Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases and cancer. Oncogene Jan 10; 24 (2) : 287-91.

Background Reference 03

Van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25 (5) :2031-44.