Parkin (phospho Ser101) Antibody

Product Specifications

Background

Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner aet al.,1992) . Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005) .

NCBI Gene ID

5071

Swiss Prot

O60260

Accession Number

O60260

Host

Rabbit

Reactivity

Human, Bovine

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human parkin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Parkin antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with bovine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Symbol

PARK2

NCBI Official Name

Parkin RBR E3 ubiquitin protein ligase

NCBI Organism

Homo sapiens

Background Reference 01

Jenner P, Dexter DT, Sian J, Schapira AH, Marsden CD (1992) Oxidative stress as a cause of nigral cell death in Parkinson’s disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7.

Background Reference 02

Yamamoto A, Friedlein A, Imai Y, Takahashi R, Kahle PJ, Haass C (2005) Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol chem. 280 (5) :3390-9