MEK5 (phospho Ser311 / Thr315) Antibody

Product Specifications

Background

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999) . An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003) . Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004) .

NCBI Gene ID

29568

Swiss Prot

Q62862

Accession Number

Q62862

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish, Bovine, Chicken, Frog

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

MEK5 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, chicken, bovine, canine, non-human primate, Xenopus, and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Symbol

Map2k5

NCBI Official Name

Mitogen activated protein kinase kinase 5

NCBI Organism

Rattus norvegicus

Background Reference 01

Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270 (21) :12665-9.

Background Reference 02

Chao TH, Hayashi M, Tapping RI, Kato Y, Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway. J. Biol. Chem. 274 (51) :36035-8.

Background Reference 03

Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostrate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22 (9) :1381-9.