5 Lipoxygenase (phospho Ser523) Antibody

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001) . Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004) . Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004) . Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Drazen JM, Lilly CM, Sperling R, Rubin P, Israel E (1994) Role of cysteinyl leukotrienes in spontaneous asthmatic responses. Adv. Prostaglandin Thromboxane Leukot Res 22:251-262.

Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M (2004) Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. New England J Med 350:29-37.