Raf-1 (phospho Ser642) Antibody

Product Specifications

Background

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996) . Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003) . Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.

NCBI Gene ID

24703

Swiss Prot

P11345

Accession Number

P11345

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Bovine, Chicken

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Raf-1 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, and non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Symbol

Raf1

NCBI Official Name

V-raf-leukemia viral oncogene 1

NCBI Organism

Rattus norvegicus

Background Reference 01

Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.

Background Reference 02

Dhillon AS, Kolch W (2002) Untying the regulation of the Raf-1 kinase. Arch Biochem Biophys 404:3-9.

Background Reference 03

Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK (2005) Regulation of Raf-1 by Direct Feedback Phosphorylation. Mol Cell 17:215-224.