Rabphilin (phospho Ser234) Antibody

Product Specifications

Background

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994) . The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005) . Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001) .

NCBI Gene ID

171039

Swiss Prot

P47709

Accession Number

P47709

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of rabphilin 3A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Rabphilin antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Symbol

Rph3a

NCBI Official Name

Rabphilin 3A

NCBI Organism

Rattus norvegicus

Background Reference 01

Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.

Background Reference 02

Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.

Background Reference 03

Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond) 369:493-497.