Aquaporin 2 (phospho Ser261) Antibody

Product Specifications

Background

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007) . A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002) . Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007) .

NCBI Gene ID

25386

Swiss Prot

P34080

Accession Number

P34080

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Bovine, Chicken

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Affinity Purified

Concentration

Batch dependent

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Aquaporin 2 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

29/37

Applications Notes

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Symbol

Aqp2

NCBI Official Name

Aquaporin 2 (collecting duct)

NCBI Organism

Rattus norvegicus

Background Reference 01

Van Balkom BW, Savelkoul PJ, Markovich D, Hofman E, Nielsen S, van der Sluijs P, Deen PM (2002) The role of putative phosphorylation sites in the targeting and shuttling of the aquaporin 2 water channel. J Biol Chem 277 (44) :41473-9.

Background Reference 02

Ford P, Rivarola V, Chara O, Blot-Chabaud M, Cluzeaud F, Farman M, Parisi M, Capurro C (2005) Volume regulation in cortical collecting duct cells: role of AQP2. Biol Cell 97 (9) :687-97.

Background Reference 03

Hoffert JD, Nielsen J, , Yu MJ, , Pisitikun T, , Schleicher SM, , Nielsen Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. . Am J Physiol Renal Physiol 292: F691-F700..