Dopamine Transporter Antibody

Product Specifications

Background

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999) . Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996) .

NCBI Gene ID

6531

Swiss Prot

Q01959

Accession Number

Q01959

Host

Rabbit

Reactivity

Human

Immunogen

Peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Affinity Purified

Concentration

Batch dependent

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Dopamine Transporter antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

Calculated Molecular Weight

Applications Notes

The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.

Symbol

SLC6A3

NCBI Official Name

Solute carrier family 6 (neurotransmitter transporter), member 3

NCBI Organism

Homo sapiens

Background Reference 01

Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.

Background Reference 02

Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.

Background Reference 03

Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.