Stargazin Antibody

Stargazin is a member of the transmembrane AMPAR regulatory proteins (TARP) family and is involved in glutamate receptor trafficking. It has been recently demonstrated (Tomita et al., 2005; Priel et al., 2005) that the interaction between stargazin and AMPA receptors is critical for the correct localization of the receptors at the synapse. Phosphorylation of the stargazin protein at Thr 321 by Protein Kinase A regulates its interaction with PSD-95 and synaptic targeting of AMPA receptors (Choi et al., 2002) .

The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Tomita S, Adesnik H, Sekiguchi M, Zhang W, Wada K, Howe JR, Nicoll RA, Bredt DS, (2005) Stargazin modulates AMPA receptor gating and trafficking by distinct domains. Nature 435: 1052-1058.

Priel A, Kolleker A, Ayalon G, Gillor M, Osten P, Stern-Bach Y. (2005) Stargazin reduces desensitization and slows deactivation of the AMPA-type glutamate receptors. J. Neurosci. 25, 2682–2686.

Choi, J, Ko J, Park E, Lee JR, Yoon J, Lim S, Kim E, Phosphorylation of stargazin by protein kinase A regulates its interaction with PSD-95.J Biol Chem. 2002 Apr 5;277 (14) :12359-63.