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WASP Antibody

Product Specifications

NCBI Gene ID

7454

Swiss Prot

P42768

Accession Number

NP_000368.1

Host

Goat

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SPADKKRSGKKKI

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Positive Control

Cat. No. 1215 - U937 Whole Cell Lysate

Concentration

500 ug/mL

Homology

Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

No cross-reactivity expected with N WASP (WASL) .

Applications Notes

Peptide ELISA: IHC:Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.Flow Cytometry:

Symbol

WAS

NCBI Official Name

Wiskott-Aldrich syndrome

NCBI Organism

Homo sapiens

Background Reference 01

Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 2005 Jan 31;168 (3) :441-52.

Other Product Names

WAS, WASP, Wiskott-Aldrich syndrome (eczema-thrombocytopenia), THC, IMD2, thrombocytopenia 1 (X-linked), Wiskott-Aldrich syndrome (eczema-thrombocytopenia) protein, THC1, Wiskott-Aldrich syndrome protein

Tested Applications

ELISA, IHC, IF, Flow

Protein ID

4507909

Physical Properties

Liquid

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