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UBCH10 Antibody

Product Specifications

NCBI Gene ID

11065

Swiss Prot

O00762

Accession Number

NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1, NP_001268670.1

Host

Goat

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QETYSKQVTSQEP

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.

Applications Notes

Peptide ELISA: Western Blot:Immunofluorescence: Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunoprecipitation:

Symbol

UBE2C

NCBI Official Name

Ubiquitin-conjugating enzyme E2C

NCBI Organism

Homo sapiens

Background Reference 01

Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Proc Natl Acad Sci U S A 1997 Mar 18;94 (6) :2362-7

Other Product Names

UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1

Tested Applications

ELISA, WB, IF, Flow, IP

Protein ID

5902146

Physical Properties

Liquid

Frequently Asked Questions

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