UBCH10 Antibody
Product Specifications
NCBI Gene ID
11065
Swiss Prot
O00762
Accession Number
NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1, NP_001268670.1
Host
Goat
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QETYSKQVTSQEP
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=46-544
Specificity
This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Applications Notes
Peptide ELISA: Western Blot:Immunofluorescence:
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry:
Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
Immunoprecipitation:
Symbol
UBE2C
NCBI Official Name
Ubiquitin-conjugating enzyme E2C
NCBI Organism
Homo sapiens
Background Reference 01
Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Proc Natl Acad Sci U S A 1997 Mar 18;94 (6) :2362-7
Other Product Names
UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
Tested Applications
ELISA, WB, IF, Flow, IP
Protein ID
5902146
Physical Properties
Liquid
Curated Selection
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