FHL1 Antibody

Product Specifications

NCBI Gene ID

2273

Swiss Prot

Q13642

Accession Number

NP_001440.2, NP_001153171.1

Host

Goat

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: CNKRFVFHNEQVY

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Positive Control

Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

Concentration

500 ug/mL

Homology

Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1) . Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Applications Notes

Peptide ELISA: Western Blot:Immunohistochemistry:Additional validation:

Symbol

FHL1

NCBI Official Name

Four and a half LIM domains 1

NCBI Organism

Homo sapiens

Background Reference 01

Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem. 1999 Sep 17;274 (38) :27083-91.

Other Product Names

FHL1, SLIM1, KYO-T, RP11-535K18.1, FHL1A, four and a half LIM domains 1, Four-and-a-half LIM domains 1, skeletal muscle LIM-protein1, FHL1B, KYOT, MGC111107, XMPMA, SLIMMER, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein1