IL-33 Antibody [IL33305B]

Product Specifications

Background

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3) /ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human IL-33.

Clonality

Monoclonal

Clone

IL33305B

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Chemokines & Cytokines

Purification

Protein G-affinity purified.

Concentration

1 mg/ml

Buffer

Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2aκ

Specificity

Recognizes human IL-33. Does not cross-react with mouse IL-33.

Applications Notes

ELISA: (direct or indirect: 1:2'000-1:5'000), Functional Application (Blocking), Immunohistochemistry (paraffin sections (1:50-1:200) ), Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.