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Calreticulin Antibody

Product Specifications

Background

Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.

NCBI Gene ID

811

Swiss Prot

P27797

Accession Number

4757900

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human calreticulin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Immunology, Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 6 months after receipt when stored at -20˚ C.

Specificity

Recognizes human calreticulin. Detects a band of ~60kDa by Western blot.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Symbol

CALR

NCBI Official Name

Calreticulin

NCBI Organism

Homo sapiens

Other Product Names

CRP55; Calregulin; Endoplasmic Reticulum Resident Protein 60; ERp60; grp60

Tested Applications

ELISA, WB

Protein ID

NP_004334

Physical Properties

Liquid

Frequently Asked Questions

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