Calreticulin Antibody
Product Specifications
Background
Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.
NCBI Gene ID
811
Swiss Prot
P27797
Accession Number
4757900
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human calreticulin.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Immunology, Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 6 months after receipt when stored at -20˚ C.
Specificity
Recognizes human calreticulin. Detects a band of ~60kDa by Western blot.
Applications Notes
ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Symbol
CALR
NCBI Official Name
Calreticulin
NCBI Organism
Homo sapiens
Other Product Names
CRP55; Calregulin; Endoplasmic Reticulum Resident Protein 60; ERp60; grp60
Tested Applications
ELISA, WB
Protein ID
NP_004334
Physical Properties
Liquid
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