FGF-21 Antibody
Product Specifications
Background
FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) . Activity requires the presence of bKlotho.
NCBI Gene ID
26291
Swiss Prot
Q9NSA1
Accession Number
9506597
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Recombinant human FGF-21.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 6 months after receipt when stored at -20˚ C.
Specificity
Recognizes human and mouse FGF-21. Detects a band of ~25kDa by Western blot.
Applications Notes
ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Symbol
FGF21
NCBI Official Name
Fibroblast growth factor 21
NCBI Organism
Homo sapiens
Other Product Names
Fibroblast Growth Factor 21
Tested Applications
ELISA, IHC, WB
Protein ID
NP_061986.1
Physical Properties
Liquid
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