Resistin Antibody

Product Specifications

Background

The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.

NCBI Gene ID

57264

Swiss Prot

Q99P87

Accession Number

12667798

Host

Rabbit

Reactivity

Mouse

Immunogen

Recombinant mouse resistin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 6 months after receipt when stored at -20˚ C.

Specificity

Recognizes mouse resistin. Detects a band of ~10kDa by Western blot.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:10,000) . Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.