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Adiponectin Antibody

Product Specifications

Background

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

NCBI Gene ID

246253

Swiss Prot

Q8K3R4

Accession Number

81901098

Host

Rabbit

Reactivity

Rat

Immunogen

Recombinant rat adiponectin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 6 months after receipt when stored at -20˚ C.

Specificity

Recognizes rat adiponectin. Weakly cross-reacts with mouse adiponectin. Detects a band of ~35kDa by Western blot.

Applications Notes

ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunohistochemistry: (frozen sections (1:1,000) ) . Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Symbol

Adipoq

NCBI Official Name

Adiponectin, C1Q and collagen domain containing

NCBI Organism

Rattus norvegicus

Other Product Names

ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa

Tested Applications

ELISA, IHC, WB

Protein ID

Q8K3R4

Physical Properties

Liquid

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