Adiponectin Antibody

Product Specifications

Background

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

NCBI Gene ID

11450

Swiss Prot

Q60994

Accession Number

408359957

Host

Rabbit

Reactivity

Mouse

Immunogen

Recombinant mouse adiponectin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 6 months after receipt when stored at -20˚ C.

Specificity

Recognizes mouse adiponectin. Weakly cross-reacts with rat adiponectin. Detects a band of ~35kDa by Western blot.

Applications Notes

ELISA: (direct and indirect; 1:2,000-1:10,000) . Immunoprecipitation: (1-5 ul per sample in a final reaction volume of 0.5-1 ml) . Western Blot: (1:2,000-1:10,000 using ECL; suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature) . Optimal conditions must be determined individually for each application.