BimS/EL/L Antibody [10B12]
Product Specifications
Background
Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3) . It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X (L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.
Host
Rat
Reactivity
Dog, Human, Monkey, Mouse, Rat
Immunogen
Synthetic peptide corresponding to aa 20-40 of mouse BimL.
Clonality
Monoclonal
Clone
10B12
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
>95% (SDS-PAGE)
Concentration
1 mg/ml
Buffer
Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=36-186
Fragment
IgG2a, kappa
Specificity
Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa) . Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.
Applications Notes
ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Other Product Names
BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death
Tested Applications
ELISA, Flow, ICC, IHC, IP, WB
Physical Properties
Liquid
Curated Selection
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