BimS/EL/L Antibody [10B12]

Product Specifications

Background

Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3) . It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X (L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.

Host

Rat

Reactivity

Dog, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 20-40 of mouse BimL.

Clonality

Monoclonal

Clone

10B12

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

>95% (SDS-PAGE)

Concentration

1 mg/ml

Buffer

Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2a, kappa

Specificity

Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa) . Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.