Apaf-1 Antibody [13F11]
Product Specifications
Background
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
NCBI Gene ID
78963
Swiss Prot
Q9EPV5
Accession Number
20137192
Host
Rat
Reactivity
Mouse, Rat
Immunogen
Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Clonality
Monoclonal
Clone
13F11
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
>95% (SDS-PAGE)
Concentration
1 mg/ml
Buffer
Liquid. In PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Fragment
IgG2a, kappa
Specificity
Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
Applications Notes
Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
Symbol
Apaf1
NCBI Official Name
Apoptotic peptidase activating factor 1
NCBI Organism
Rattus norvegicus
Other Product Names
Apoptosis Protease-activating Factor 1
Tested Applications
ELISA, ICC, IP, WB
Protein ID
Q9EPV5
Physical Properties
Liquid
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