Apaf-1 Antibody [13F11]

Product Specifications

Background

Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

NCBI Gene ID

78963

Swiss Prot

Q9EPV5

Accession Number

20137192

Host

Rat

Reactivity

Mouse, Rat

Immunogen

Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Clonality

Monoclonal

Clone

13F11

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

>95% (SDS-PAGE)

Concentration

1 mg/ml

Buffer

Liquid. In PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=36-176

Fragment

IgG2a, kappa

Specificity

Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.

Applications Notes

Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Symbol

Apaf1

NCBI Official Name

Apoptotic peptidase activating factor 1

NCBI Organism

Rattus norvegicus

Other Product Names

Apoptosis Protease-activating Factor 1

Tested Applications

ELISA, ICC, IP, WB

Protein ID