Apaf-1 Antibody [2E12]

Product Specifications

Background

Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

NCBI Gene ID

317

Swiss Prot

O14727

Accession Number

2330015

Host

Rat

Reactivity

Human

Immunogen

Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Clonality

Monoclonal

Clone

2E12

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

>95% (SDS-PAGE)

Concentration

1 mg/ml

Buffer

Liquid. In PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2a, kappa

Specificity

Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Applications Notes

Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000) ) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.