NS5B Antibody [5B-12B7]
Product Specifications
Background
The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
Host
Mouse
Reactivity
HCV
Immunogen
Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
Clonality
Monoclonal
Clone
5B-12B7
Conjugation
Unconjugated
Type
Primary Antibodies, Functional Antibodies
Field of Research
Innate Immunity
Purification
>95% (SDS-PAGE)
Concentration
1 mg/ml
Buffer
Liquid. In PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Fragment
IgG2a
Specificity
Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392) . Does not react with genotype 2.
Applications Notes
Western Blot: (1-2ug/ml) . Optimal conditions must be determined individually for each application.
Other Product Names
Nonstructural Protein 5B
Tested Applications
Functional Assays, ICC, IP
Physical Properties
Liquid
Frequently Asked Questions
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