FTO Antibody [AG103]

Product Specifications

Background

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

NCBI Gene ID

79068

Swiss Prot

Q9C0B1

Accession Number

166851822

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human FTO.

Clonality

Monoclonal

Clone

AG103

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2a, kappa

Specificity

Recognizes human FTO.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunoprecipitation: (5ug/ml per sample in a final reaction volume of 500-1,000ul) . Immunohistochemistry: (5-10ug/ml: paraffin sections) . Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.