Calreticulin Antibody [CR213-2AG]

Product Specifications

Background

Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.

NCBI Gene ID

811

Swiss Prot

P27797

Accession Number

4757900

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human calreticulin.

Clonality

Monoclonal

Clone

CR213-2AG

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Immunology, Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human calreticulin.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.