Sirtuin 2 Antibody [S2R233-1]

Product Specifications

Background

Sirtuin-2 is a NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of a-tubulin. It is involved in the control of mitotic exit in the cell cycle. Furthermore, a variety of age-associated diseases such as type 2 diabetes, obesity, osteoporosis, and Alzheimer’s disease are associated with sirtuins. Selective inhibition of Sirtuin-2 achieves neuroprotection and identifies Sirtuin-2 to be a potential therapeutic avenue in Huntington’s and Parkinson’s disease.

NCBI Gene ID

22933

Swiss Prot

Q8IXJ6

Accession Number

13775600

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human sirtuin 2.

Clonality

Monoclonal

Clone

S2R233-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Innate Immunity, Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2b, kappa

Specificity

Recognizes human sirtuin 2. Detects a band of ~45kDa by Western blot.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.