FGF-23 Antibody [FG322-3]

Product Specifications

Background

FGF-23 is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC) .

NCBI Gene ID

8074

Swiss Prot

Q9GZV9

Accession Number

10190674

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human FGF-23.

Clonality

Monoclonal

Clone

FG322-3

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human FGF-23. Does not cross-react with mouse FGF-23.

Applications Notes

ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections (1:100-1:500) ) . Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.