DLK1 Antibody [PF299-1]
Product Specifications
Background
DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.
NCBI Gene ID
8788
Swiss Prot
P80370
Accession Number
74136023
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human DLK1 (extracellular domain) .
Clonality
Monoclonal
Clone
PF299-1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=36-071
Fragment
IgG1, kappa
Specificity
Recognizes human DLK1. Does not cross-react with mouse DLK1.
Applications Notes
ELISA: (direct or indirect: 1:2,000-1:10,000) . Flow Cytometry: (1:100-1:500) . Immunohistochemistry: (paraffin sections (1:100-1:500) ) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.
Symbol
DLK1
NCBI Official Name
Delta-like 1 homolog (Drosophila)
NCBI Organism
Homo sapiens
Other Product Names
Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Tested Applications
ELISA, Flow, IHC, WB
Protein ID
NP_003827
Physical Properties
Liquid
Curated Selection
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