DLK1 Antibody [PF13-3]

Product Specifications

Background

DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.

NCBI Gene ID

8788

Swiss Prot

P80370

Accession Number

74136023

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human DLK1 (extracellular domain) .

Clonality

Monoclonal

Clone

PF13-3

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human DLK1. Does not cross-react with mouse DLK1.

Applications Notes

ELISA: (direct or indirect: 1:2,000-1:10,000) . Flow Cytometry: (1:100-1:500) . Immunohistochemistry: (1:200-1:1,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.