FGF-19 Antibody [FG98-6]

Product Specifications

Background

FGF-19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. FGF-19 plays an important role in brain development during embryogenesis. FGF-19 binds only to FGF receptor 4 (FGFR4) . Activity requires the presence of KLB.

NCBI Gene ID

9965

Swiss Prot

O95750

Accession Number

11132196

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human FGF-19.

Clonality

Monoclonal

Clone

FG98-6

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2b, kappa

Specificity

Recognizes human FGF-19. Detects a band of ~25kDa by Western blot.

Applications Notes

ELISA: (direct or indirect: 1:500-1:1,000), Immunohistochemistry: (paraffin sections, 15ug/ml) . Western blot: (1:500-1:1,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.