FTO Antibody [FT86-4]

Product Specifications

Background

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

NCBI Gene ID

79068

Swiss Prot

Q9C0B1

Accession Number

166851822

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human FTO.

Clonality

Monoclonal

Clone

FT86-4

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human, mouse and rat FTO.

Applications Notes

ELISA: (direct or indirect: 1:2,000-1:5,000) . Immunoprecipitation: (1-5ul per sample in a final reaction volume of 500-1000ul) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.