DLK1 Antibody [PF105B]

Product Specifications

Background

DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.

NCBI Gene ID

13386

Swiss Prot

Q09163

Accession Number

1169354

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant mouse DLK1 (extracellular domain) .

Clonality

Monoclonal

Clone

PF105B

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Concentration

~1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG2a, kappa

Specificity

Recognizes mouse DLK1. Does not cross-react with human DLK1.

Applications Notes

ELISA: (direct or indirect: 1:2,000-1:10,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.