Jagged-1 Antibody [J1G53-3]

Product Specifications

Background

Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro) . Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF) .

NCBI Gene ID

182

Swiss Prot

P78504

Accession Number

4557679

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Jagged-1.

Clonality

Monoclonal

Clone

J1G53-3

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human Jagged-1. Detects a band of ~180kDa by Western blot.

Applications Notes

ELISA: (direct or indirect: 1:500-1:5,000) . Flow Cytometry: (1:20-1:100) . Immunohistochemistry: (paraffin sections) . Western blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.