Ribosomal Protein S3 Antibody [RP159-1]

Product Specifications

Background

RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-kappaB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3.

NCBI Gene ID

6188

Swiss Prot

P23396

Accession Number

417719

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human ribosomal protein S3.

Clonality

Monoclonal

Clone

RP159-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human ribosomal protein S3. Detects a band of ~32kDa by Western blot.

Applications Notes

ELISA: (direct or indirect: 1:500-1:5,000) . Western blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.