ST2 Antibody [ST33868]

Product Specifications

Background

The ST2 gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form (ST2) and a longer, transmembrane form (ST2L) by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.

NCBI Gene ID

9173

Swiss Prot

Q01638

Accession Number

116242527

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human soluble ST2.

Clonality

Monoclonal

Clone

ST33868

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human ST2.

Applications Notes

ELISA: (direct or indirect: 1:2,000-1:5,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.