ANGPTL4 Antibody [Kairos-1]

ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced) . Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD) . Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.

Recognizes the fibrinogen-like domain (FLD) of human ANGPTL4. Detects a band of ~62kDa and ~35kDa by Western blot. Does not cross-react with other ANGPTL family proteins.

ELISA: (direct or indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections (1:100-1:1,000) ) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions should be determined individually for each application.