Adiponectin Antibody [RADI 264]
Product Specifications
Background
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
NCBI Gene ID
246253
Swiss Prot
Q8K3R4
Accession Number
81901098
Host
Mouse
Reactivity
Rat
Immunogen
Recombinant rat adiponectin.
Clonality
Monoclonal
Clone
RADI 264
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=36-037
Fragment
IgG1, kappa
Specificity
Recognizes rat adiponectin. Detects a band of ~30kDa by Western blot. Does not cross-react with human or mouse adiponectin.
Applications Notes
ELISA: (direct or indirect: 1:5,000-1:10,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.
Symbol
Adipoq
NCBI Official Name
Adiponectin, C1Q and collagen domain containing
NCBI Organism
Rattus norvegicus
Other Product Names
ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Tested Applications
ELISA, WB
Protein ID
Q8K3R4
Physical Properties
Liquid
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