Adiponectin Antibody [RADI 264]

Product Specifications

Background

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

NCBI Gene ID

246253

Swiss Prot

Q8K3R4

Accession Number

81901098

Host

Mouse

Reactivity

Rat

Immunogen

Recombinant rat adiponectin.

Clonality

Monoclonal

Clone

RADI 264

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes rat adiponectin. Detects a band of ~30kDa by Western blot. Does not cross-react with human or mouse adiponectin.

Applications Notes

ELISA: (direct or indirect: 1:5,000-1:10,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.