IDO Antibody [ID 177]

Product Specifications

Background

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

NCBI Gene ID

3620

Swiss Prot

P14902

Accession Number

4504577

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human IDO.

Clonality

Monoclonal

Clone

ID 177

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Immunology, Innate Immunity, Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested.

Applications Notes

ELISA: (direct or indirect: 1:2,000-1:10,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.